Last year over one million pints of Help for Heroes, a collaborative ale from Marston's and injured veterans, were sold.
Its success means it will return for a second year this month.
The 4.2 per cent ABV blonde ale raised £110,000 for the charity in 2015 and again this year, 6p from every pint sold will be donated to the cause.
"Help for Heroes is dedicated to supporting wounded members of our Armed Forces for life and due to the help we were able to give the charity by selling their bespoke beer last year, it felt only right that we continued on this journey," said the brewer's senior drinks category manager, Mark Carter.
"We look forward to an even more successful 2016."
The beer will be available in Marston's pubs until the beginning of March when it will be rolled out the wider trade.
Marston's will also be running a range of charity events across its 1,000-strong pub estate to raise more funds and is organising a "Walk for Heroes" for head office staff from its Burton brewery to Park brewery in Wolverhampton.