A special edition of the beer, inspired by Mexico's Dia de los Muertos (Day of the Dead) celebrations, has been released.
Halloween is now the third biggest sales period for the on-trade, according to CGA data, after Christmas and Bank Holidays.
The rock band's Eddie character will don a traditional Dia de los Muertos costume through October and November to tap into what brewer Robinsons says is a growing thirst for Halloween beers.
There'll be limited edition pump clips for the cask version of the brew as well as a temporary new look for the bottled version.
The launch of the special packs coincides with Iron Maiden's sell-out Legacy of the Beast world tour with stadium shows in Mexico, Brazil, Chile and Argentina, in September and October.
Trooper brand manager, Sam Kennerley, said: "The Halloween opportunity in the UK keeps growing and we wanted to create something a little different this year to give Trooper fans and beer lovers something new to engage with."
Since its launch in 2013, Trooper has sold over 25 million pints worldwide.